|
| Status |
Public on Feb 10, 2017 |
| Title |
Liver tissue from adult pig taken at slaughter LPA DL Le 2873 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Adult German Landrace
loin eye area (lea) [cm2]: 45.9
fat area (fa) [cm2]: 12.6
meat to fat ratio (mfr): 0.27
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
biotin-labeled cRNAs were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42 C on Affymetrix Porcine Snowball Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
Gene expression data from liver
loin eye area (LEA) [cm2]: area of M. longissimus dorsi (Mld) at 13th/14th rib
fat area (FA) [cm2]: fat area on Mld at 13th/14th rib
meat to fat ratio (MFR): ratio of meat and fat area
MV = missing value
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
|
| |
|
| Submission date |
Jun 30, 2016 |
| Last update date |
Feb 10, 2017 |
| Contact name |
Siriluck Ponsuksili |
| E-mail(s) |
[email protected]
|
| Organization name |
FBN
|
| Street address |
Wilhelm-stahl-allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16569 |
| Series (1) |
| GSE83932 |
Gene expression data of mRNA from liver of pig breed German Landrace |
|
