|
| Status |
Public on Aug 15, 2007 |
| Title |
lymphocytes_HD_M_55y |
| Sample type |
RNA |
| |
|
| Source name |
lymphocytes (blood)
|
| Organism |
Homo sapiens |
| Characteristics |
Sample Group: HD patient; Gender: male; Age: 55; Tissue: lymphocytes (blood); IT15 CAG repeats: 25/43
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol (Invitrogen) following the manufacturer's protocol, followed by RNeasy column cleanup and ethanol precipitation.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufacturer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Description |
Affymetrix U133 Human Genome 2.0 Plus arrays
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Aug 13, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Alexandre Kuhn |
| E-mail(s) |
[email protected]
|
| Organization name |
NIH
|
| Department |
NIA
|
| Lab |
Laboratory of Neurogenetics
|
| Street address |
35 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-3707 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8762 |
Lymphocyte gene expression data from moderate stage HD patients and controls |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
