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| Status |
Public on Dec 30, 2017 |
| Title |
oz9 |
| Sample type |
SRA |
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| Source name |
ventral rumen wall
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| Organism |
Ovis aries |
| Characteristics |
age: adult
sample: oz9
block: 4
liveweight (kg): 71
ch4 g/d: 15.5838625
dry matter intake g/d: 746.687
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
qualities were checked and fastq files were sent by Ramaciotti Centre for Genomics
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Ovis aries3.1 whole genome using bowtie2 and TopHat with defualt parameters
counts (exon based) were calculated by HTSeq and were normalised by EdgeR VOOM
Supplementary_files_format_and_content: tab-delimited text files include EdgeR VOOM normalised values for each sample
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| Submission date |
May 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruidong Xiang |
| E-mail(s) |
[email protected]
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| Organization name |
CSIRO
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| Street address |
Level 5, QBP, 306 Carmody rd,
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| City |
St lucia |
| State/province |
QLD |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platform ID |
GPL15670 |
| Series (1) |
| GSE81847 |
RNA seq of ventral rumen wall of Australian sheep |
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| Relations |
| BioSample |
SAMN05172503 |
| SRA |
SRX1798629 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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