|
| Status |
Public on Feb 12, 2017 |
| Title |
PDL36 |
| Sample type |
RNA |
| |
|
| Source name |
TIG3 cells passaging until PDL36
|
| Organism |
Homo sapiens |
| Characteristics |
derived cell: TIG3
cell state: proliferating
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with ReliaPrep RNA Cell MiniPrep System (Promega) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Starting with 200 ng of the isolated RNA for each sample, double stranded cDNA and cyanine 3 labeled cRNA were synthesized using low input quick amp labeling kit, one-color and RNA spike-in kit, one-color (Agilent).
|
| |
|
| Hybridization protocol |
The resultant cRNAs were fragmented for 30 min at 60°C in dark. The fragmented cRNAs (600 ng) were hybridized on the microarray at 65°C for 17 h.
|
| Scan protocol |
After washing, slides were scanned with the Agilent microarray scanner G2505B.
|
| Data processing |
Feature extraction software (version 10.7.3.1) was used to calculate signal intensities from the scanned images.
|
| |
|
| Submission date |
May 23, 2016 |
| Last update date |
Feb 12, 2017 |
| Contact name |
Mizuho Sakaki |
| E-mail(s) |
[email protected]
|
| Organization name |
National Research Institute for Child Health and Development
|
| Department |
Maternal-Fetal Biology
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE81786 |
Potential roles of DNA methylation in the initiation and establishment of replicative senescence revealed by array-based methylome and transcriptome analyses [expression] |
| GSE81798 |
Potential roles of DNA methylation in the initiation and establishment of replicative senescence revealed by array-based methylome and transcriptome analyses |
|
