|
| Status |
Public on Aug 03, 2007 |
| Title |
Gene expression in mutant zebrafish larvae at 72 hours postfertilization |
| Sample type |
RNA |
| |
|
| Source name |
Mutant zebrafish larvae at 72 hours postfertilization
|
| Organism |
Danio rerio |
| Characteristics |
RNA obtained from mutant zebrafish larvae at 72 hours postfertilization
|
| Biomaterial provider |
Han Wang, PhD, University of Oklahoma
|
| Treatment protocol |
Total RNA was obtained from approximately 50 larvae post-fertilization
|
| Growth protocol |
Embryos were growed at 28.5 0C according to a standard procedure (Westerfield. 2000. The zebrafish book)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Direct labeling incorporating Cy3-dUTP in cDNA from 2 micrograms total RNA with anchored oligo-dT priming and Omniscript reverse transcriptase
|
| |
|
| Hybridization protocol |
The labeled cDNA was added to ChipHybeTM hybridization buffer (Ventana Medical Systems, Tucson) containing Cot-1 DNA (0.5 mg/ml), yeast tRNA (0.2 mg/ml), and poly(dA)40-60 (0.4 mg/ml). Hybridization was performed on a Ventana Discovery system for 9 hr at 58 0C
|
| Scan protocol |
Agilent DNA Microarray Scanner Model G2505B with fluorescent intensity was measured by Imagene™ software
|
| Description |
None
|
| Data processing |
’associative analysis' method of Dozmorov & Centola, Bioinformatics 19:204-211, 2003. PubMed ID (PMID): 12538240
|
| |
|
| Submission date |
Aug 01, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Han Wang |
| E-mail(s) |
[email protected]
|
| Phone |
4053259324
|
| Fax |
4053253442
|
| URL |
http://www.ou.edu/wanglab
|
| Organization name |
University of Oklahoma
|
| Department |
Zoology/Stephenson Research and Technology Center
|
| Lab |
Zebrafish Genetics and Genomics Laboratory
|
| Street address |
101 David L. Boren Boulevard
|
| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73019 |
| Country |
USA |
| |
|
| Platform ID |
GPL5692 |
| Series (1) |
| GSE8651 |
Transcriptome analysis of zebrafish yquem/urod mutant |
|
