|
| Status |
Public on Apr 23, 2016 |
| Title |
Z138 shBTK |
| Sample type |
SRA |
| |
|
| Source name |
mantle cell lymphoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Z138 stable cell transfected with small hairpin targeting BTK, and induced with Doxycyclin for 48H
|
| Growth protocol |
Mantle cells were grown in RPMI1640 media plus 20%FBS,1% penicillin, 1% streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Rneasy plus mini kit (Qiagen) following the manufacturer’s instructions.
cDNA library was construbuted by using Illumina Truseq RNA Library Prep Kit (RS-122-2002)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RNAseq reads were filtered for average QC score>30. Reads were mapped to hg19 genome.
RNAseq fpkm were generated by tophat and cufflink by following Trapnell et al.(2012).
Supplementary_files_format_and_content: RNA-seq (txt file: gene fpkm)
|
| |
|
| Submission date |
Apr 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Xuehua Zhong |
| Organization name |
Wisconsin Institute for Discovery
|
| Department |
Epigenetics
|
| Street address |
330 N. Orchard St.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE80563 |
FBXO10 deficiency and BTK activation upregulate BCL2 expression in mantle cell lymphoma |
|
| Relations |
| BioSample |
SAMN04884740 |
| SRA |
SRX1719797 |
