|
| Status |
Public on Jul 25, 2016 |
| Title |
IL8-3 20DAF rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Tomato introgression line 'IL8-3' fruit, 20 day after flowering, replicate 3
|
| Organism |
Solanum lycopersicum |
| Characteristics |
cultivar: IL8-3
tissue: Fruit pericarp
developmental stage: 20 day after flowering
|
| Growth protocol |
M82 and IL8-3 plants were grown in a planting bed with culture soil containing slow-release fertilizer (CDU TamagokaseiS555, Co-op Chemical) and granular dolomite (Magutan, Gausukogyo) in a greenhouse, as described previously (Ikeda et al. 2013). Fruits were sampled at 10, 20, 30 DAF, and ripening stage (breaker stage) from 8 plants. Pericarp tissues were frozen in liquid nitrogen and stored at −80°C¬.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fruit by the SDS–phenol method (Mohammed et al. 2012) and further purified with an RNeasy Plant Mini Kit (Qiagen). The optical density of the RNA was measured to check the quality, and high-quality RNA was subjected to transcriptome analysis using three independent biological replicates.
|
| Label |
Cy3
|
| Label protocol |
Target labeling was performed according to the manual of the Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manual of the Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies). Following hybridization, we washed the probe array.
|
| Scan protocol |
We scanned tomato custom-designed microarray images with Agilent DNA Microarray Scanner G2565CA (Agilent Technologies).
|
| Description |
Gene expression of IL8-3 fruit
|
| Data processing |
Scanned microarray images were converted to signal data with Feature Extraction software (Agilent Technologies).
|
| |
|
| Submission date |
Feb 24, 2016 |
| Last update date |
Jul 25, 2016 |
| Contact name |
Yoshinori Kanayama |
| E-mail(s) |
[email protected]
|
| Phone |
+81-22-717-8642
|
| Organization name |
Tohoku University
|
| Department |
Graduate School of Agricultural Science
|
| Lab |
Laboratory of Horticultural Science
|
| Street address |
1-1, Tsutsumidori-amamiyamachi, Aobaku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
981-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21511 |
| Series (1) |
| GSE78260 |
Transcriptome analysis of tomato introgression line during fruit development. |
|
