|
| Status |
Public on Feb 19, 2009 |
| Title |
mRNA quantitation of failed heart |
| Sample type |
RNA |
| |
|
| Source name |
19 weeks Dahl rat with a high-sodium diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
LV myocytes isolated from Dahl rat with a failed heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNeasy mini spin column (Qiagen, Inc).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Ovation Biotin RNA Amplification and Labeling System, NuGEN Technologies, Inc.
|
| |
|
| Hybridization protocol |
2 ug of cDNA were hybridized for 18 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChip arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
LV myocytes of CHF rat was hybridized.
|
| Data processing |
The mean value for the internal control probe sets (defined by Affymetrix) was set to be 500.
|
| |
|
| Submission date |
Jun 30, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Hiroyuki Mano |
| E-mail(s) |
[email protected]
|
| Phone |
+81-285-58-7449
|
| Fax |
+81-285-44-7322
|
| Organization name |
Jichi Medical University
|
| Department |
Division of Functional Genomics
|
| Street address |
3311-1 Yakushiji
|
| City |
Shimotsukeshi |
| State/province |
Tochigi |
| ZIP/Postal code |
329-0498 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE8341 |
ChIP-on-chip experiments of normal and failed hearts of Dahl rat |
|
