|
| Status |
Public on Feb 29, 2016 |
| Title |
mutant 2 CXCR4 transfected human hematopoetic leukemic k562 cells replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
N119S CAM CXCR4 gene transfected human hematopoetic leukemic k562 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: K562
transfection: constitutively active mutant N119S CXCR4 gene
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNA isolation kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
The samples for Gene expression were labeled using Agilent Quick-Amp labeling Kit (p/n5190-4444).
|
| |
|
| Hybridization protocol |
The labeled cRNA samples were hybridized on to a Custom Whole Genome Human 8x60k designed by Genotypic Technology Pvt. Ltd. (AMADID: 27114)
|
| Scan protocol |
Scanned with GenePix scanner having 5µm resolution in a single pass scanning mode having a scan area of 61 * 21.6mm.
|
| Description |
induced and exponentially growing
Biological replicate 2 of 3. constitutively active N119S CXCR4 mutants transfected human hematopoetic leukemic k562 cells.
|
| Data processing |
The data was normalized by percentile shift normalization method using GeneSpring GX11 (Agilent technologies, India). Following normalization, gProcessed signal (dye normalized background subtracted signal intensity) of the probes showing at least 30% change in expression across the 3 donors were computed and ratios (treated/untreated) were log2 transformed. Statistical analysis of the data was performed using Cyber-T regularized t statistic due to small sample size (n= 3) since it takes into account Bayesian estimate of variance by pooling across genes with similar intensities.
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| |
|
| Submission date |
Jan 05, 2016 |
| Last update date |
Feb 29, 2016 |
| Contact name |
Gurudutta U Gangenahalli |
| E-mail(s) |
[email protected]
|
| Phone |
9101123905144
|
| Organization name |
Institute of Nuclear Medicine & Allied Sciences
|
| Department |
Stem Cell Research Division
|
| Lab |
Stem Cell & Gene Therapy Research Group
|
| Street address |
Brig. S. K. Mazumdar Marg, Timarpur
|
| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110054 |
| Country |
India |
| |
|
| Platform ID |
GPL13252 |
| Series (1) |
| GSE76544 |
Human hematopoietic leukemic k562 cells: control Vs CXCR4 constitutively active mutant cells |
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