|
| Status |
Public on Jun 29, 2007 |
| Title |
Terpestacin treatment in HT1080 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HT1080 human fibrosarcoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
For Ch1, RNA was prepared from untreated HT1080 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TriZol and then cleaned using QIAGEN RNeasy mini kit.
|
| Label |
Cy3
|
| Label protocol |
Fluorescently labeled cDNA for cDNA microarray analysis was prepared by the reverse transcription of total RNA in the presence of aminoallyl-dUTP followed by coupling of the Cy3 dye
|
| |
|
| Channel 2 |
| Source name |
HT1080 human fibrosarcoma cell line treated with 20microM terpestacin
|
| Organism |
Homo sapiens |
| Characteristics |
For Ch2, RNA was prepared from HT1080 cells treated with 20micromolar terpestacin for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TriZol and then cleaned using QIAGEN RNeasy mini kit.
|
| Label |
Cy5
|
| Label protocol |
Fluorescently labeled cDNA for cDNA microarray analysis was prepared by the reverse transcription of total RNA in the presence of aminoallyl-dUTP followed by coupling of the Cy5 dye
|
| |
|
| |
| Hybridization protocol |
Hybridization
- the DNA chip was hybridized with a mixture of fluorescently labeled cDNAs from control and treated cells at 58 °C for 16 hr
|
| Scan protocol |
Scan Protocol
- the DNA chip was scanned using ScanArray Lite (Perkin-Elmer Life Sciences, Billerica, MA).
|
| Description |
None
|
| Data processing |
Data processing
- The raw data was normalized by LOWESS normalization method.
|
| |
|
| Submission date |
May 29, 2007 |
| Last update date |
Jun 01, 2007 |
| Contact name |
Hye Jin Jung |
| E-mail(s) |
[email protected]
|
| Phone |
82-2-2123-5883
|
| Fax |
82-2-362-7265
|
| Organization name |
Yonsei University
|
| Department |
Biotechnology
|
| Lab |
Chemical Genomics
|
| Street address |
134 Shinchon-dong,Seodaemun-gu
|
| City |
Seoul |
| ZIP/Postal code |
120-749 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL1818 |
| Series (1) |
| GSE7953 |
Correlation analysis of genome-wide gene expression profiles by DNA microarray |
|
