|
| Status |
Public on Oct 27, 2024 |
| Title |
L-AOPP 50µM 2h, IPyA 100nM 1h (085) |
| Sample type |
RNA |
| |
|
| Source name |
L-AOPP 50µM 2h, then treated with IPyA 100nM 1h
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedling
treatment: L-AOPP 50µM 2h, then treated with IPyA 100nM 1h
|
| Treatment protocol |
The seedlings were treated with or without 50 µM AOPP for 3 h. Alternatively, the seedlings were treated with AOPP for 2 h, and then treated with or without 1 µM IAA or IPyA for additional 1 h.
|
| Growth protocol |
Four-day-old Arabidopsis seedlings were grown at 22 degrees under continuous light.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
guanidine-hydrochloride method
|
| Label |
biotin
|
| Label protocol |
One-cycle target labeling reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix Gene Array Scanner 2500A with the original setting (This means that scanning steps are conducted at a high level of sensitivity to focus on those genes that function in hormone signaling, because their transcripts are relatively less abundant. Under these conditions, signals of highly abundant transcripts may reach saturation)
|
| Description |
L-AOPP 50µM 2h→IPyA 100nM 1h
|
| Data processing |
MAS5.0 signal
|
| |
|
| Submission date |
Nov 19, 2015 |
| Last update date |
Oct 27, 2024 |
| Contact name |
Yukihisa Shimada |
| E-mail(s) |
[email protected]
|
| Organization name |
Yokohama City University
|
| Department |
Kihara Institute for Biological Science
|
| Lab |
Plant Biotechnology Division
|
| Street address |
Maiokacho, Totsuka
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
244-0813 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE75182 |
Gene expression response to L-aminooxyphenylpropionic acid (AOPP) in Arabidopsis |
|
