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Sample GSM1888507 Query DataSets for GSM1888507
Status Public on Jun 08, 2016
Title Input ChIP-Seq Replicate 3
Sample type SRA
 
Source name Yeast
Organism Cryptococcus neoformans
Characteristics strain: N/A
cell density: OD=2.5
Growth protocol 50 ODs of cells from overnight YPAD cultures were washed once in water and resuspended in 20 mLs DMEM, transferred to 150mm tissue culture dishes, and incubated for 24 hours at 37ÂșC, 5% CO2
Extracted molecule genomic DNA
Extraction protocol mRNA was isolated using trizol to isolate total RNA and oligotex mRNA mini kit to isolate mRNA. ChIP DNA was isolated from antibody incubation by SDS/proteinase K followed by purification using MN Nucleospin Gel Cleanup columns
cDNA libraries were prepared from DNase-treated mRNA using NEBNext Ultra Directional RNA Library Prep Kit. ChIP DNA libraries were prepared using End-It DNA End Repair Kit followed by A-tailing using Klenow fragment (NEB), ligation to Illumina adaptors using Rapid T4 DNA Ligase (Enzymatics), PCR amplification for 15 cycles, and size selection between 200-350bp
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
 
Data processing ChIP-Seq data were aligned using Bowtie1, allowing up to two mismatches within the seed sequence. Indexed, sorted bam files were created for each dataset using SAMtools and bedgraph files were created using BEDTools.
Peaks in ChIP-Seq data were called using MACS with a manually set shift-size calculated using the spp package.
Motif calling was performed using Gimsan with default settings.
Genome_build: Sequence and feature files for C. neoformans var grubii H99 were obtained from the Broad institute, Cambridge, MA as current as January 20th, 2011.
Supplementary_files_format_and_content: Peak lists and locations for ChIP-seq samples and RPKM tables for RNA-seq samples.
 
Submission date Sep 18, 2015
Last update date May 15, 2019
Contact name Christina M. Homer
E-mail(s) [email protected]
Organization name UCSF
Department Biochemistry & Biophysics
Lab Madhani Lab
Street address 600 16th St
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL19081
Series (1)
GSE73203 Convergent evolution of peptide-based quorum sensing required for virulence in a eukaryotic pathogen
Relations
BioSample SAMN04096352
SRA SRX1261434

Supplementary file Size Download File type/resource
GSM1888507_input_rep3_peaks.txt.gz 60 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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