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Sample GSM1874180 Query DataSets for GSM1874180
Status Public on Oct 15, 2018
Title Control 1
Sample type SRA
 
Source name Foxo1f/f, uterus
Organism Mus musculus
Characteristics age: 8 weeks
strain: C57BL/6J
genotype/variation: Foxo1f/f
developmental stage: Pseudo pregnancy day 4.5
tissue: uterus
Treatment protocol Control Foxo1f/f and conditional knock out PgrCre/+ Foxo1f/f female mated with vasectomized males. Uteri harvest at day 4.5 of pseudopregnancy. Serum progesterone levels used to confirm stage of pregnancy.
Growth protocol standard chow
Extracted molecule total RNA
Extraction protocol RNA was isolated using the Qiagen RNeasy Kit
Illumina TruSeq RNA Sample Preparation Protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description FD41834
Data processing In order to increase the mappability, we trimmed 11 low-quality nucleotides from the 5’ ends of the reads.
The resulting 90-nucleotide pair-ended reads were mapped to the mouse genome (UCSC mm9) using STAR (Dobin et al., 2013) with NCBI RefSeq genes as the reference.
In order to reduce possible PCR biases, we removed the read duplicates using picard tools (http://broadinstitute.github.io/picard/)
HTseq (http://www-huber.embl.de/users/anders/HTSeq) was used to determine the number of reads falling in the known genes.
EdgeR (Robinson et al., 2010) was used to analyze the gene-based read counts to detect differentially expressed genes between the control and treatment groups.
Genome_build: mm9
Supplementary_files_format_and_content: EdgeR_KO.vs.WT_DE.txt reports Symbol, rawMeanCount_WT, rawMeanCount_KO, FC_edgeR, log2CPM_edgeR, PV_edgeR, FDR_edgeR, EntrezGene, geneName2.
 
Submission date Sep 10, 2015
Last update date May 15, 2019
Contact name Rainer B Lanz
E-mail(s) [email protected]
Phone 7137986478
Organization name Baylor College of Medicine
Department Molecular and Cellular biology
Street address One Baylor Plaza
City Houaton
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL17021
Series (2)
GSE72895 FOXO1 Regulates Uterine Epithelial Integrity and Progesterone Receptor Expression Critical for Embryo Implantation [RNA-seq]
GSE72896 FOXO1 Regulates Attachment of the Implanting Blastocyst to the Endometrium at the Window of Receptivity
Relations
BioSample SAMN04045418
SRA SRX1219627

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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