|
| Status |
Public on Sep 01, 2016 |
| Title |
tumor metformin 1 |
| Sample type |
RNA |
| |
|
| Source name |
tumor, 2mg metformin
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: tumor
disease type: cholangiocarcinoma
|
| Treatment protocol |
Tumor: The metformin-treated group was injected 5 times a week intraperitoneally (i.p.) at 2 mg/body per day for 31 days.
|
| Growth protocol |
Tumor (mouse): Each mouse was inoculated with HuCCT-1 cells (5×106cells/animal) subcutaneously on the flank regions of the mouse.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Quiagen).
|
| Label |
Hy5
|
| Label protocol |
Extracted total RNA was labeled with Hy5 using the miRCURY LNA Array miR labeling kit (Exiqon, Vedbaek, Denmark)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction (Toray Industries Inc., Tokyo, Japan).
|
| Description |
2mg-1
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Aug 10, 2015 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15446 |
| Series (1) |
| GSE71905 |
Study of microRNA associated with metformin’s anti-tumor effect in cholangiocarcinoma |
|
