|
| Status |
Public on Jul 18, 2017 |
| Title |
TO_132_13June08 |
| Sample type |
RNA |
| |
|
| Source name |
blood cells
|
| Organism |
Vulpes lagopus |
| Characteristics |
tissue: blood
|
| Biomaterial provider |
The University of Washington, Seattle, WA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All blood samples destined for molecular analyses were collected in PAXgene (Qiagen) RNA tubes and stored on ice in the field until they were placed in -20°C or -80°C freezers. RNA was extracted using PaxGene Blood miRNA Kit (Qiagen) and quantity and integrity determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) or a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific).
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Canine Genome 2.0 Array through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics & Proteomics Facility Core Laboratory in the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/members/core-services.html).
|
| Description |
blood cells
|
| Data processing |
Affymetrix Expression Console, RMA normalization log2
|
| |
|
| Submission date |
Jul 27, 2015 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE71407 |
Using Domestic and Free-Ranging Arctic Canid Models for Environmental Molecular Toxicology Research |
|
