|
| Status |
Public on Jul 11, 2015 |
| Title |
THP1 5 hours DMSO treatment replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
THP1 acute myeloid leukemia cell line
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: acute myeloid leukemia cell line
cell line: THP-1
treatment: DMSO
|
| Treatment protocol |
RNA was isolated from THP-1 cells after 5 hours of treatment with IPA-3 (6 ug/mL), FRAX-597 (2 ug/mL) or an equal volume of DMSO using Trizol Reagent (Invitrogen).
|
| Growth protocol |
THP-1 cells were purchased from ATCC and cultured according to ATCC recommendations (RPMI-1640 + 10%FCS + P/S). Cells were maintained in an incubator at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled with the GeneChip WT terminal labeling kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Labeled cRNA of each individual sample was hybridized to Affymetrix Human Gene 2.0ST microarrays (Affymetrix)
|
| Scan protocol |
Scanned by GeneChip Scanner 3000 7G system (Affymetrix) according to standard protocols.
|
| Description |
Gene expression data from THP1 cells treated with DMSO for 5 hours.
|
| Data processing |
Affymetrix Expression Console Software and Transcriptome Analysis Console
|
| |
|
| Submission date |
Jul 10, 2015 |
| Last update date |
Jul 11, 2015 |
| Contact name |
Ashley M Pandolfi |
| E-mail(s) |
[email protected]
|
| Phone |
7184303437
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Lab |
Ulrich Steidl
|
| Street address |
1300 Morris Park Chanin 606
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE70791 |
PAK1 is a Therapeutic Target in Acute Myeloid Leukemia and Myelodysplastic Syndrome |
|
