|
| Status |
Public on Dec 29, 2015 |
| Title |
cKO_1 |
| Sample type |
RNA |
| |
|
| Source name |
cKO mouse 733
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: DMP1 Cre Mbtps1 knockout
tissue: soleus muscles
age: 39-41 weeks
gender: male
|
| Treatment protocol |
39-41 week old male mice were euthanized with carbon dioxide gas and then soleus muscles were removed from each leg and flash frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from frozen tissues using RNEasy protocol with an oncolumn DNase step. The RNA was checked for quality using a nanodrop and a bioanalyzer nanochip (Agilent).
|
| Label |
biotin
|
| Label protocol |
100ng total RNA was converted to fragmented biotinylated cDNA using the standard WT Plus protocol (Affymetrix)
|
| |
|
| Hybridization protocol |
labeled samples were hybridized to Mouse Transcriptome1.0 Array (Affymetrix) using standard protocol
|
| Scan protocol |
The arrays were scanned on a GCS3000 7G scanner using standard Affymetrix protocols
|
| Data processing |
The CEL files were converted to CHP files using Expression Consolev 1.4. Further statistical analysis was done using Affymetrix Transcriptome Console v3 and GeneSpring GXv13
|
| |
|
| Submission date |
Jun 18, 2015 |
| Last update date |
Dec 29, 2015 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
[email protected]
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platform ID |
GPL20258 |
| Series (1) |
| GSE69985 |
Microarray on mouse soleus muscles from DMP1 Cre Mbtps1 knockout mice compared with littermate controls |
|
