|
| Status |
Public on Dec 01, 2018 |
| Title |
Muscle stem cells cultured in 10% FBS and 100 ng/mL oncostatin M for 3 days |
| Sample type |
RNA |
| |
|
| Source name |
MuSCs_10% FBS and 100 ng/mL oncostatin M for 3 days
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: wildtype
age: 8 weeks
tissue: muscle
cell type: cultured muscle stem cells (MuSC)
culture condition: 10% FBS and 100 ng/mL oncostatin M for 3 days
|
| Treatment protocol |
Muscle stem cells were cultured in 10% FBS plus or minus 100 ng/mL oncostatin M for 3 days
|
| Growth protocol |
Mouse muscle stem cells were purified essentially as previously described (Cosgrove et al., 2014) using a FACS Aria 2, routinely yielding >95% CD34+α7 integrin+ cells from a single round of sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared with Qiagen RNeasy kits according to Qiagen's instructions
|
| Label |
biotin
|
| Label protocol |
Twenty nanograms of total RNA was prepared for array analysis using Affymetrix 3’ IVT Express amplification reagents
|
| |
|
| Hybridization protocol |
Fifteen micrograms of cRNA was hybridized to Affymetrix MOE_430_2 arrays, and arrays were washed and stained using an Affymetrix FS4500 fluidics station
|
| Scan protocol |
GeneChips were scanned using The Affymetrix GC3000 scanner
|
| Description |
MuSC_plus_OSM
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
|
| |
|
| Submission date |
Jun 18, 2015 |
| Last update date |
Dec 01, 2018 |
| Contact name |
John R Walker |
| E-mail(s) |
[email protected]
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE69976 |
Induction of muscle stem cell quiescence by the secreted niche factor Oncostatin M |
|
