|
| Status |
Public on Oct 31, 2015 |
| Title |
number 157 pig heart |
| Sample type |
RNA |
| |
|
| Source name |
pig heart in control group
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: heart
phenotype: normal
diet: normal diet
|
| Treatment protocol |
The pigs were fasted for 12 hours and euthanized with ketamine and xylazine. Pig hearts were sampled and preserved in liquid nitrogen.
|
| Growth protocol |
The control group consisted of 6 Bama pigs fed a control diet, and the HFHSD group comprised 6 pigs that were induced with a HFHS diet, which included 37% sucrose, 53% control diet and 10% pork lard for 23 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from control group
Bama minipig_157
|
| Data processing |
Slides were scanned by GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings. Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
|
| |
|
| Submission date |
Apr 14, 2015 |
| Last update date |
Oct 31, 2015 |
| Contact name |
Jihan Xia |
| E-mail(s) |
[email protected]
|
| Phone |
861062818180
|
| Organization name |
Chinese academy of agrigultural sciences
|
| Street address |
No. 2 Yuanmingyuan West Road, Beijing,
|
| City |
beijing |
| State/province |
beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE67890 |
Expression data from Bama minipig hearts - high-fat, high-sucrose diet |
|
