|
| Status |
Public on Oct 26, 2015 |
| Title |
COLO205_DMSO_2H_D |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Colo205 colon cancer cells were treated with DMSO for 2H
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Colo205
treatment: DMSO
time: 2 hr
|
| Treatment protocol |
The Colo205 colon cancer cell line was treated for 2 or 6 hours with vehicle control (DMSO) or treated with Compound 1
|
| Growth protocol |
All cell lines were purchased from the American Type Culture Collection and grown in their recommended culture medium, which was supplemented with 10% fetal bovine serum (FBS) at 37°C in an atmosphere of 5% CO2 and passaged for less than six months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the ABI PRISM 6100 Nucleic Acid PrepStation (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturer’s instructions
|
| Label |
Cy3
|
| Label protocol |
Quick Amp Labeling protocol
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Reference
|
| Treatment protocol |
The Colo205 colon cancer cell line was treated for 2 or 6 hours with vehicle control (DMSO) or treated with Compound 1
|
| Growth protocol |
All cell lines were purchased from the American Type Culture Collection and grown in their recommended culture medium, which was supplemented with 10% fetal bovine serum (FBS) at 37°C in an atmosphere of 5% CO2 and passaged for less than six months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the ABI PRISM 6100 Nucleic Acid PrepStation (Applied Biosystems, Carlsbad, CA, USA) according to the manufacturer’s instructions
|
| Label |
Cy5
|
| Label protocol |
Quick Amp Labeling protocol
|
| |
|
| |
| Hybridization protocol |
Cy 3 labelled cRNA was co-hybridized with Cy5 labelled Universal Human Reference RNA
|
| Scan protocol |
The arrays were scanned using an Agilent Technologies Scanner G2505C US94400327, using the XDR function (extended dynamic range at 100% and 10%) The images were feature extracted to generate raw data files using Agilent feature extraction version 10.7.3.1 and extraction protocol GE2_107_Sep09 and grid file 039494_D_F_20120628
|
| Description |
COLO205_DMSO_2H_D.txt
|
| Data processing |
Background subtracted data for sample and reference were entered in the Genespring analysis software (Agilent). Lowess normalization was applied to the samples.
|
| |
|
| Submission date |
Apr 14, 2015 |
| Last update date |
Oct 26, 2015 |
| Contact name |
Robert te Poele |
| E-mail(s) |
[email protected]
|
| Phone |
00442087224319
|
| Organization name |
The Institute of Cancer Research
|
| Department |
Cancer Reserach UK Unit for Cancer Therapeutics
|
| Lab |
Signal Transduction and Molecular Pharmacology Team
|
| Street address |
15 Cotswold Road
|
| City |
Sutton |
| State/province |
Surrey |
| ZIP/Postal code |
SM2 5NG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE67845 |
Identification of a potent and selective chemical probe for exploring the role of Mediator complex-associated protein kinases CDK8 and CDK19 in human disease [Colo205] |
| GSE67849 |
Identification of a potent and selective chemical probe for exploring the role of Mediator complex-associated protein kinases CDK8 and CDK19 in human disease |
|
