|
| Status |
Public on Feb 19, 2016 |
| Title |
WTCorticalNeuronsNeurite2 |
| Sample type |
SRA |
| |
|
| Source name |
WT E18.5 cortical neurons neurite
|
| Organism |
Mus musculus |
| Characteristics |
cell type: E18.5 cortical neurons
compartment: neurite
genotype: wild type
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cells were fractionated using porous membranes into soma and neurite fractions.
For all N2A and CAD samples, libraries were prepared using the standard "dUTP" strand-specific method. For cortical neuron samples, libraries were prepared using the Clontech Low Input Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
WT cortical neurons neurite Rep2
|
| Data processing |
Reads were aligned to the mm9 genome using either Tophat version 2.0.12 or STAR 2.0.4d
Expression values were calculated using Cufflinks version 2.1.1
Alternative isoform abundances were calculated using MISO
Genome_build: mm9 supplemented with alternative isoforms identified in Merkin et al (Science) 2012
|
| |
|
| Submission date |
Apr 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew Taliaferro |
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Biology
|
| Street address |
12801 E 17th Ave, RC1 South Room 10403D
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE67828 |
Profiling of soma and neurite transcriptomes |
|
| Relations |
| BioSample |
SAMN03481300 |
| SRA |
SRX993480 |
