|
| Status |
Public on Feb 26, 2016 |
| Title |
Pax7-/- ESCs day 21, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
embryoid body outgrowths at day 21 of differentiation_Pax7-/-
|
| Organism |
Mus musculus |
| Characteristics |
strain: F1 (C57Bl6N x 129Sv)
genotype: Pax7-/-
cells: embryoid body outgrowths
|
| Treatment protocol |
Material was callected at day 0 (undeifferentiated ESCs) and at day 7 and 21 of differentiation. Cells were washed with PBS and frozen in -80C.
|
| Growth protocol |
ESCs were cultured under standard conditions, i.e. KO-DMEM containing all necessary supplements and LIF. Embryoid bodies and embryoid body outgrowths were cultured in KO-DMEM containing all necessary supplements, lacking LIF and supplemented with RA and ITS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using High Pure RNA isolation kit (Roche) according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
According to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
According to manufacturer's protocol.
|
| Scan protocol |
The chips were scanned using the Affymetrix GeneAtlas scanner according to manufacturer's protocol.
|
| Description |
Gene expression data
|
| Data processing |
The microarray gene expression data was analyzed using Partek Genomic Suite 6.6 software (Partek Inc., Louis, MO). Raw data (.cel files) were imported and processed, using Robust Multiarray Averaging (RMA) background correction, quantile normalization, Log2 transformation and median polish summarization.
|
| |
|
| Submission date |
Mar 03, 2015 |
| Last update date |
Feb 27, 2016 |
| Contact name |
Areta Magda Czerwinska |
| Organization name |
University of Warsaw
|
| Department |
Faculty of Biology
|
| Lab |
Department of Cytology
|
| Street address |
Krakowskie Przedmiescie 26/28
|
| City |
Warsaw |
| ZIP/Postal code |
00-096 |
| Country |
Poland |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE66483 |
Expression data from differentiating mouse embryonic stem cells wild type and lacking functional Pax7 gene |
|
