|
| Status |
Public on Nov 23, 2015 |
| Title |
1086-084-Y5 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
pid: 1086_084
psi: 23
lag1psi: 23
chronicpsi: 0
age: 59
female: 0
hispanic: 0
black: 0
married: 1
lnincome: 11.3794
bmi: 33.0428
smoke: 1
alcohol: 1
cesd: 0
pss: 1
socsupp: 15.3333
monocytepct: Missing
cd3d: 12.2193
cd3e: 10.001
cd4: 9.676
cd8a: 10.229
cd19: 10.7808
ncam1: 7.1559
fcgr3a: 6.7413
cd14: 13.6597
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Primary peripheral blood mononuclear cells were isolated and total RNA was extracted using Qiagen RNEasy.
|
| Label |
biotin
|
| Label protocol |
Ambion TotalPrep RNA amplification with biotin labeling for gene expression profiling (carried out by UCLA Neuroscience Genomics Core).
|
| |
|
| Hybridization protocol |
Illumina cRNA hybridization protocol for gene expression profiling (carried out by UCLA Neuroscience Genomics Core).
|
| Scan protocol |
Standard Illumina scan protocol for gene expression profiling (carried out by UCLA Neuroscience Genomics Core).
|
| Description |
raw data file column: 5384103013_E.AVG_Signal
Illumina Human Ref-8 BeadArray
|
| Data processing |
Raw probe intensity values and quantile-normalized gene expression values were generated by Illumina GenomeStudio software using Gene Expression module 1.1.1. All available data are provided, with no additional filtering.
|
| |
|
| Submission date |
Jan 22, 2015 |
| Last update date |
Nov 23, 2015 |
| Contact name |
Steve Cole |
| Organization name |
UCLA School of Medicine
|
| Department |
Medicine
|
| Street address |
11-934 Factor Bldg, UCLA
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1678 |
| Country |
USA |
| |
|
| Platform ID |
GPL10399 |
| Series (1) |
| GSE65213 |
Leukocyte gene expression as a function of perceived social isolation (PSI): Year 5 |
|
