|
| Status |
Public on Nov 03, 2015 |
| Title |
PB_300ppm_PB_15B |
| Sample type |
RNA |
| |
|
| Source name |
300ppm_PB
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
gender: male
exposed to: 300ppm phenobarbital (PB)
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
300ppm_PB_REP2_15B
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 15, 2014 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Susan Hester |
| E-mail(s) |
[email protected]
|
| Phone |
919-541-1320
|
| Organization name |
US EPA
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE64187 |
Dose and Effect Thresholds for Early Key Events in a PPARĪ± Tumorigenic Mode of Action |
|
