|
| Status |
Public on Nov 27, 2007 |
| Title |
Liver phenobarbital pool3 Affy |
| Sample type |
RNA |
| |
|
| Source name |
liver, phenobarbital treated
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: male
Age: 9 - 12 weeks
Tissue: liver
Treatment: 50 mg/kg of phenobarbital
|
| Biomaterial provider |
Adrian Roth
|
| Treatment protocol |
Animals were injected i.p. 50 mg/kg of phenobarbital in vehicle (5% DMSO in corn oil). After 10h they were sacrificed.
|
| Growth protocol |
Animals had free acess to food (standard laboratory chow) and drinking water. They were mantained in 12-hour light/12-hour dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer protocol and purified by RNeasy Mini Kit (Qiagen). The total RNAs of two animals were pooled in equal amounts.
|
| Label |
biotin
|
| Label protocol |
All was done according to the Affymetrix recommendations. Briefly, double-stranded cDNA was synthesised using 10mg of total RNA with a (dT)24-T7 primer and amplified with T7 RNA polymerase in the presence of biotinylated nucleotides to cRNA.
|
| |
|
| Hybridization protocol |
After fragmentation, the cRNA was hybridised to the Gene Chips for 16 hours, followed by a standard amplification with biotin/streptavidin/phycoerythrin.
|
| Scan protocol |
MIcroarrays were scanned using a laser scanner (Affymetrix).
|
| Description |
No additional data.
|
| Data processing |
Raw data were normalized using the Robust Multichip Average (RMA) algorithm as described. After transformation to non-logarithmic data, the expression estimates were scaled to the average expression levels in the control group analyzed using GeneSpring software (Silicon Genetics, Redwood, USA).
|
| |
|
| Submission date |
Jan 12, 2007 |
| Last update date |
Jan 30, 2007 |
| Contact name |
Peter Juvan |
| Phone |
+386 1 543 7595
|
| Organization name |
Faculty of Medicine, University of Ljubljana
|
| Department |
Institute of Biochemistry
|
| Lab |
Center for Functional Genomics and Bio-Chips
|
| Street address |
Zaloska 4
|
| City |
Ljubljana |
| ZIP/Postal code |
SI-1000 |
| Country |
Slovenia |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6721 |
High cholesterol diet and phenobarbital affect cholesterol homeostasis and drug metabolism |
|
