|
| Status |
Public on Dec 31, 2015 |
| Title |
cardiac muscle newborn knockout repl.3 |
| Sample type |
RNA |
| |
|
| Source name |
heart, neonate,KO
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cardiac muscle
age: newborn
genotype/variation: knockout
strain: C57/Bl6
|
| Treatment protocol |
not applicable
|
| Growth protocol |
mouse ventricular muscle dissected from P1-2 and P30 mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
hearts were scised, minced and washed in ice-cold buffered saline and inmediatly frozen. Kept at -80ºC until RNA extraction
|
| Label |
biotin
|
| Label protocol |
Samples 5μg of ssDNA were fragmented and labeled using the Terminal Labeling kit (Affymetrix) following protocol guides.Approximately 3μg of fragmented and labeled ssDNA was hybridzed per array using the Samples 5μg of ssDNA were fragmented and labeled using the Terminal Labeling kit (Affymetrix) following protocol guides.
|
| |
|
| Hybridization protocol |
Approximately 3μg of fragmented and labeled ssDNA was hybridzed per array using the Affymetrix hybridization guides. Arrays were hybridized for 17 h in GeneTitan Affymetrix platform
|
| Scan protocol |
Arrays were scanned on GeneTitan Affymetrix platform
|
| Data processing |
Data were processed using the limma (v3.14) and oligo (v1.22) packages in the R (v2.15) environment. Probeset level expression was derived using Robust Multi-array Average normalization. Non-specific filtering of 50% less intense and variable data was applied. Data were limited to core probe set only, and summarized at the gene expression level.
probe group file: MoGene-1_1-st-v1.r4.pgf
meta-probeset file: MoGene-1_1-st-v1.r4.mps
|
| |
|
| Submission date |
Nov 05, 2014 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Daniel Sanchis |
| E-mail(s) |
[email protected]
|
| Organization name |
IRBLLEIDA - University of LLeida
|
| Department |
Basic Medical Sciences
|
| Lab |
b2.13
|
| Street address |
Av. Rovira Roure, 80
|
| City |
Lleida |
| ZIP/Postal code |
25198 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE63023 |
Expression data from heart muscle of cardiac-specific caspase-3 and -7 knockout and wild type newborn and young mice |
|
