|
| Status |
Public on Dec 31, 2016 |
| Title |
hHP-NUP98-HOXA9 clone 2 |
| Sample type |
RNA |
| |
|
| Source name |
Hematopoietic progenitors (CD34+) from cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue/cell type: Hematopoietic progenitors (CD34+) from cord blood
|
| Growth protocol |
Human Hematopoietic Progenitors (hHP; CD34+) were purified using immunomagnetic beads (Miltenyi Biotech, Germany) from human umbilical cord blood (CB). They were cultured in Iscove’s modified Dulbecco’s media (IMDM) containing 20% BIT 9500 Serum substitute (STEMCELL Technologies), 1% penicillin-streptomycin (Invitrogen), 0.2% β-mercaptoethanol, 10 ng/ml SCF, megakaryocyte growth and development factor (TPO), FLT3 ligand (FLT3L), IL-3, and IL-6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer's recommendations.
|
| Label |
Cy5
|
| Label protocol |
0.9 ug total RNA was labeled with cy3-CTP following Agilent Low Input Quick Amplification Labeling Kit protocol for One-Color Microarray-Based Gene Expression Analysis
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufacturer’s protocol (Agilent One-Color Microarray-Based Gene Expression Analysis v6.5) using 600 ng cy3-labeled cRNA per sample and SurePrint G3 Human Gene Expression 8x60K Microarrays.
|
| Scan protocol |
Scanned on an G2505C DNA microarray scanner (Agilent).
|
| Description |
Gene expression
|
| Data processing |
Images were analysed by Agilent Feature Extraction Software (ver. 11.5), which performed feature quantitation and additive detrend correction. No background subtraction
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Ana Del Rio |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Street address |
Calle Melchor Fernandez Almagro 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE62585 |
Development of NUP98-HOXA9 gene expression signatures |
| GSE62587 |
NUP98-HOXA9 role as oncogenic transcription factor |
|
