|
| Status |
Public on Oct 17, 2014 |
| Title |
Human Female low BMD_3 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy female_low_BMD
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: Peripheral blood monocytes
Sex: female
|
| Treatment protocol |
Monocyte isolation：A monocyte negative isolation kit (Order No. 130-091-153, Miltenyi Biotec Inc., Auburn, CA, USA) was used to isolate circulating monocytes from 150mL whole blood.
|
| Growth protocol |
One hundred and fifty milliliters of peripheral blood were drawn for each selected subject.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Genomic DNA was extracted from the freshly isolated PBMs. Total RNA from monocytes was extracted using a Qiagen RNeasy Mini Kit . RNA integrity was assessed by using an Agilent 2100 Bioanalyzer .
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was synthesized by using the Superscript Choice System, followed by an in vitro transcription reaction with a T-7 (dT24) primer to produce biotinylated cRNA. The full-length cRNAs were fragmented to 20 to 200 bp and hybridized to Affymetrix GeneChip Human Exon 1.0 ST Array. 100 ng of total RNA was amplified and labeled using the Affymetrix Whole-Transcript (WT) Sense Target Labeling Protocol without rRNA reduction.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Human Exon 1.0 ST arrays were hybridized with 11 μg of labeled sense DNA.
|
| Scan protocol |
Array scanning was performed according to the protocol described in WT Sense Target Labeling Assay Manual
|
| Data processing |
All the expression data of the 10 samples had been preprocessed using robust multichip average (RMA) normalization.
|
| |
|
| Submission date |
Oct 16, 2014 |
| Last update date |
Oct 17, 2014 |
| Contact name |
shishi min |
| E-mail(s) |
[email protected]
|
| Phone |
15580884581
|
| Organization name |
Hunan Normal University
|
| Department |
College of life sciences
|
| Lab |
Laboratory of Molecular and Statistical Genetics
|
| Street address |
Lu shan road
|
| City |
Chang sha |
| State/province |
Hunan |
| ZIP/Postal code |
410000 |
| Country |
China |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE62402 |
Integrative Analysis of Transcriptomic and Epigenomic Data to Reveal Regulation Patterns for Osteoporosis Risk [Transcriptome] |
| GSE62589 |
Integrative Analysis of Transcriptomic and Epigenomic Data to Reveal Regulation Patterns for Osteoporosis Risk |
|
