NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM150300 Query DataSets for GSM150300
Status Public on Dec 15, 2006
Title rab3a-/- hippocampus hrm5A
Sample type RNA
 
Source name hippocampus
Organism Mus musculus
Characteristics rab3a-/- hippocampus
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description hippocampus from rab3a-/- mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL339
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 97.5 P 0.004484
AFFX-BioB-M_at 167.5 P 0.000225
AFFX-BioB-3_at 51.8 P 0.001796
AFFX-BioC-5_at 215.3 P 0.00034
AFFX-BioC-3_at 155.1 P 0.000127
AFFX-BioDn-5_at 168.4 P 0.000044
AFFX-BioDn-3_at 1139.5 P 0.000169
AFFX-CreX-5_at 1838.1 P 0.000044
AFFX-CreX-3_at 2697.4 P 0.000044
AFFX-DapX-5_at 12.8 A 0.131361
AFFX-DapX-M_at 13.1 A 0.195266
AFFX-DapX-3_at 10.4 A 0.574038
AFFX-LysX-5_at 0.8 A 0.9273
AFFX-LysX-M_at 1.8 A 0.852061
AFFX-LysX-3_at 13.8 P 0.015183
AFFX-PheX-5_at 1.4 A 0.937071
AFFX-PheX-M_at 1.4 A 0.897835
AFFX-PheX-3_at 5 A 0.737173
AFFX-ThrX-5_at 5.6 A 0.60308
AFFX-ThrX-M_at 9.8 A 0.41138

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM150300.CEL.gz 3.4 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap