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Sample GSM150290

Query DataSets for GSM150290

Status

Public on Dec 15, 2006

Title

rab3a+/+ hippocampus hrw1A

Sample type

RNA

Source name

hippocampus

Organism

Mus musculus

Characteristics

rab3a+/+ hippocampus

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

hippocampus from rab3a+/+ mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL339

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	76.4	P	0.00762
AFFX-BioB-M_at	140.3	P	0.000509
AFFX-BioB-3_at	48.8	P	0.000581
AFFX-BioC-5_at	227.1	P	0.000225
AFFX-BioC-3_at	148.9	P	0.000052
AFFX-BioDn-5_at	154.8	P	0.000044
AFFX-BioDn-3_at	1136.1	P	0.000147
AFFX-CreX-5_at	1752.6	P	0.000044
AFFX-CreX-3_at	2646.7	P	0.000044
AFFX-DapX-5_at	4.9	A	0.41138
AFFX-DapX-M_at	12	A	0.300606
AFFX-DapX-3_at	5.3	A	0.883887
AFFX-LysX-5_at	0.8	A	0.98333
AFFX-LysX-M_at	2.4	A	0.772364
AFFX-LysX-3_at	7.3	A	0.250796
AFFX-PheX-5_at	0.7	A	0.945787
AFFX-PheX-M_at	1.6	A	0.804734
AFFX-PheX-3_at	11.3	A	0.41138
AFFX-ThrX-5_at	3.2	A	0.834139
AFFX-ThrX-M_at	4.4	A	0.617401

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150290.CEL.gz	3.3 Mb	(ftp)(http)	CEL