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Sample GSM150289 Query DataSets for GSM150289
Status Public on Dec 15, 2006
Title earlybird mutant hippocampus hem3A
Sample type RNA
 
Source name hippocampus
Organism Mus musculus
Characteristics earlybird mutant hippocampus
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description hippocampus from earlybird mutant mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL339
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 83.5 P 0.039661
AFFX-BioB-M_at 96.5 P 0.000662
AFFX-BioB-3_at 46.9 P 0.006871
AFFX-BioC-5_at 225.4 P 0.000297
AFFX-BioC-3_at 139.9 P 0.000127
AFFX-BioDn-5_at 191.3 P 0.00007
AFFX-BioDn-3_at 1019.7 P 0.000258
AFFX-CreX-5_at 2021.5 P 0.000044
AFFX-CreX-3_at 3024.3 P 0.000044
AFFX-DapX-5_at 6.4 A 0.48511
AFFX-DapX-M_at 8.8 A 0.404602
AFFX-DapX-3_at 5.3 A 0.910522
AFFX-LysX-5_at 1.5 A 0.910522
AFFX-LysX-M_at 16.7 A 0.470241
AFFX-LysX-3_at 7.1 A 0.544587
AFFX-PheX-5_at 1.1 A 0.960339
AFFX-PheX-M_at 1.8 A 0.804734
AFFX-PheX-3_at 5.5 A 0.772364
AFFX-ThrX-5_at 2.7 A 0.868639
AFFX-ThrX-M_at 4.9 A 0.5

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM150289.CEL.gz 3.3 Mb (ftp)(http) CEL

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