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Sample GSM150275

Query DataSets for GSM150275

Status

Public on Dec 15, 2006

Title

rab3a+/+ cortex crw4A

Sample type

RNA

Source name

cortex

Organism

Mus musculus

Characteristics

rab3a+/+ cortex

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

cortex from rab3a+/+ mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL339

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	103.3	P	0.004484
AFFX-BioB-M_at	124.5	P	0.000225
AFFX-BioB-3_at	53.9	P	0.002275
AFFX-BioC-5_at	250.9	P	0.000195
AFFX-BioC-3_at	158.3	P	0.00011
AFFX-BioDn-5_at	150.4	P	0.000052
AFFX-BioDn-3_at	1264.2	P	0.000195
AFFX-CreX-5_at	1966.3	P	0.000044
AFFX-CreX-3_at	2972	P	0.000044
AFFX-DapX-5_at	10.5	A	0.205732
AFFX-DapX-M_at	13.9	A	0.354453
AFFX-DapX-3_at	2.3	A	0.945787
AFFX-LysX-5_at	1.4	A	0.904333
AFFX-LysX-M_at	22.5	A	0.470241
AFFX-LysX-3_at	5.7	A	0.5
AFFX-PheX-5_at	2.7	A	0.904333
AFFX-PheX-M_at	1.9	A	0.897835
AFFX-PheX-3_at	22.4	A	0.287743
AFFX-ThrX-5_at	1.5	A	0.957038
AFFX-ThrX-M_at	11.3	A	0.631562

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150275.CEL.gz	3.4 Mb	(ftp)(http)	CEL