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Sample GSM150271 Query DataSets for GSM150271
Status Public on Dec 15, 2006
Title earlybird mutant cortex cem3A
Sample type RNA
 
Source name cortex
Organism Mus musculus
Characteristics earlybird mutant cortex
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description cortex from earlybird mutant mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL339
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 87.8 P 0.036569
AFFX-BioB-M_at 92.6 P 0.000297
AFFX-BioB-3_at 56.9 P 0.004998
AFFX-BioC-5_at 232.8 P 0.000446
AFFX-BioC-3_at 159.9 P 0.000127
AFFX-BioDn-5_at 220.2 P 0.00011
AFFX-BioDn-3_at 1243.3 P 0.000169
AFFX-CreX-5_at 2461.1 P 0.000044
AFFX-CreX-3_at 4385.9 P 0.000044
AFFX-DapX-5_at 10.8 A 0.195266
AFFX-DapX-M_at 14.2 A 0.262827
AFFX-DapX-3_at 1.9 A 0.957038
AFFX-LysX-5_at 0.7 A 0.945787
AFFX-LysX-M_at 2.7 A 0.824672
AFFX-LysX-3_at 5.4 A 0.5
AFFX-PheX-5_at 0.9 A 0.960339
AFFX-PheX-M_at 7.7 A 0.794268
AFFX-PheX-3_at 6.5 A 0.645547
AFFX-ThrX-5_at 4.4 A 0.843268
AFFX-ThrX-M_at 11.9 A 0.313723

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM150271.CEL.gz 3.4 Mb (ftp)(http) CEL

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