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Sample GSM150267 Query DataSets for GSM150267
Status Public on Dec 15, 2006
Title earlybird wildtype cortex cew2A
Sample type RNA
 
Source name cortex
Organism Mus musculus
Characteristics earlybird wildtype cortex
Extracted molecule total RNA
Extraction protocol Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description cortex from wild type littermate of earlybird mutant mouse
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Dec 14, 2006
Last update date Dec 14, 2006
Contact name Maja Bucan
E-mail(s) [email protected]
Organization name UPENN
Street address 415 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL339
Series (1)
GSE6527 Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 129.4 P 0.00844
AFFX-BioB-M_at 132.2 P 0.000147
AFFX-BioB-3_at 68.1 P 0.000754
AFFX-BioC-5_at 316 P 0.000297
AFFX-BioC-3_at 224.7 P 0.00011
AFFX-BioDn-5_at 244.3 P 0.000081
AFFX-BioDn-3_at 1486.1 P 0.000127
AFFX-CreX-5_at 3009.9 P 0.000044
AFFX-CreX-3_at 3900.2 P 0.000044
AFFX-DapX-5_at 4.7 A 0.396911
AFFX-DapX-M_at 18.8 A 0.165861
AFFX-DapX-3_at 1 A 0.978098
AFFX-LysX-5_at 1 A 0.932322
AFFX-LysX-M_at 3.8 A 0.712257
AFFX-LysX-3_at 12.2 A 0.440646
AFFX-PheX-5_at 1.1 A 0.937071
AFFX-PheX-M_at 2.9 A 0.860518
AFFX-PheX-3_at 30 A 0.5
AFFX-ThrX-5_at 2.3 A 0.941556
AFFX-ThrX-M_at 3.9 A 0.60308

Total number of rows: 22690

Table truncated, full table size 610 Kbytes.




Supplementary file Size Download File type/resource
GSM150267.CEL.gz 3.3 Mb (ftp)(http) CEL

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