|
| Status |
Public on Dec 13, 2006 |
| Title |
Haloperidol treated 4weeks AffyGC RMA rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Right brain hemisphere ,C57BL male mouse,Haloperidol treated,Orally,4 weeks
|
| Organism |
Mus musculus |
| Characteristics |
Tissue: Forebrain (left hemisphere), Strain: C57BL, Gender: Male, Age: 4weeks, Drug treatment: Typical antipsychotic Haloperidol, Dose: 1.6mg/kg/day, Route of treatment: Oral treatment-Drug added to drinking Water, Period of treatment: 4 weeks, Method of sacrificing: cervical dislocation.
|
| Treatment protocol |
Mouse was housed individually in a separate cage under a 12-h light–dark cycle and permitted food and water ad libitum. A period of acclimatization for two weeks was allowed prior to any experimental procedure. Haloperidol was added to the animal’s drinking water in a concentration equivalent to the highest human clinical dosage (i.e. 1.6 mg/kg/day) and delivered through light protected bottles. The weight and water consumption was measured weekly and the drug concentration in water was adjusted accordingly .At the end of 4 weeks animal was sacrificed by cervical dislocation and the brain was removed, dissected into left and right hemispheres and cerebellum, flash frozen in liquid nitrogen and stored at -80 C for future use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8-10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner (Agilent Technologies, Foster City, CA)..
|
| Description |
Gene expression data from the right forebrain of C57BL mouse received Haloperidol oral treatment four weeks.
|
| Data processing |
Normalized expression values were calculated from probe level data (CEL file) using the function of Robust Multi-Array expression measure using sequence information GC-RMA (Wu et al 2004, www.bepress.com/jhubiostat/paper4).Computational steps were implemented in the R-language. GCRMA and it's associated packages were provided by the open source bioconductor project.
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| |
|
| Submission date |
Dec 12, 2006 |
| Last update date |
Dec 12, 2006 |
| Contact name |
Mie A Rizig |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+44 (0)2076799436
|
| Fax |
+44 (0)2076799437
|
| Organization name |
University Collage London
|
| Department |
Mental Health Sciences
|
| Lab |
Molecular Psychiatry Lab
|
| Street address |
Windeyer Institute of Medical Sciences,46 Cleveland Street
|
| City |
London |
| ZIP/Postal code |
W1T 4JF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL81 |
| Series (2) |
| GSE6511 |
Four weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA). |
| GSE6512 |
Clozapine and Haloperidol effect on the mouse brain |
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