|
| Status |
Public on Mar 15, 2007 |
| Title |
granule neuron precursors, biological replicate 2; array B |
| Sample type |
RNA |
| |
|
| Source name |
cerebellum of six days old pups
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: Ink4c-/-;p53-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mus musculus Genome Array MOE430. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000
|
| Description |
Gene expression data from purified granule neuron precursors.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 06, 2006 |
| Last update date |
Mar 14, 2007 |
| Contact name |
Chunxu Qu |
| E-mail(s) |
[email protected]
|
| Phone |
9015952433
|
| Organization name |
St Jude Children's Research Institute
|
| Department |
Pathology
|
| Lab |
Mullighan
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE6463 |
Genetic alterations in mouse medulloblastomas and generation of tumors from cerebellar grunule neuron precursors |
|
