|
| Status |
Public on Jan 04, 2016 |
| Title |
NTg, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
splenic CD19 cells from NTg mice
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C3H/C57BL6
tissue: spleen
cell type: CD19 cells
genotype/variation: NTg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol (Invitrogen) according to manufacturer's protocol. It was further purified using PCI/CI extraction. RNA quality was assessed using Nanodrop ND1000 and RNA Nano Bioanayzer prior to use for microarray.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, Acetylated BSA (50 mg/ml), Affymetrix Eukaryotic Hybridization Controls (affymetrix) and Oligo B2, 3 nM (Affymetrix). Heat 65oC for 5 mins, 99oC for 5 mins, 45oC for 5 mins, centrifuge topr speed for 5 mins. Hybridize 16 hrs at 45o at 60 rpm. Fluidics washing is FS450_0002
|
| Scan protocol |
Affymetrix Gebe CHIP Scanner 3000 7G using AGCC
|
| Description |
gene expression data from NTg mice
NTg 1
|
| Data processing |
The data were analyzed through Bioconductor package, RMA method was used as the background-correction and normalization.
|
| |
|
| Submission date |
Aug 21, 2014 |
| Last update date |
Jan 04, 2016 |
| Contact name |
Tasneem Motiwala |
| E-mail(s) |
[email protected]
|
| Organization name |
The Ohio State University
|
| Street address |
420 W. 12th Ave
|
| City |
Columbus |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE60582 |
Gene expression profile of splenic CD19 cells from NTg, PTPROt Tg, TCL1 Tg and PTPROt/TCL1 double Tg mice |
|
