|
| Status |
Public on Nov 11, 2014 |
| Title |
OC1-KO embryo 3 |
| Sample type |
RNA |
| |
|
| Source name |
OC1-KO embryonic retina at E14
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed
tissue: retina
Stage: E14
onecut genotype: OC1-KO
|
| Treatment protocol |
Embryos were removed from the dam and retinas were isolated, then washed in 1X PBS.
|
| Growth protocol |
The original Onecut1 deficient mice were obtained and cross by us to C57Bl/6 for a few generations. The mice were described in Jacquemin P. et al, 2000 and were from R1 ES cells injected into a "Swiss" strain of mice.
Littermates were allowed to develop normally in utero until age E14, when they were harvested from the pregnant dam.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trireagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA wa amplified, biotinylated, and fragmented using the Ambion MessageAmp III RNA amplification kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Amplified RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
|
| Scan protocol |
Amplified RNA was scanned from Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
|
| Description |
e14 OC1_KO3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 19, 2014 |
| Last update date |
Nov 12, 2014 |
| Contact name |
Jeffrey M Trimarchi |
| E-mail(s) |
[email protected]
|
| Phone |
2172190210
|
| Organization name |
Iowa State University
|
| Department |
GDCB
|
| Lab |
Trimarchi
|
| Street address |
3188 Molecular Biology Building
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50014 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE57917 |
Expression data from E14.5 Onecut1 WT and KO animals |
|
