|
| Status |
Public on Dec 31, 2014 |
| Title |
D2 E17 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
DBA/2J mouse whole cerebellum
|
| Organism |
Mus musculus |
| Characteristics |
strain: DBA/2J
age: E17
tissue: whole cerebellum
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
E17 1735640022_F
biological replicate 2
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 15, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Thomas Ha |
| Organization name |
University of British Columbia
|
| Department |
CMMT
|
| Street address |
950 West 28th Avenue
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE60437 |
CbGRiTS: Cerebellar Gene Regulation in Time and Space |
|
