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Sample GSM1479313 Query DataSets for GSM1479313
Status Public on Nov 04, 2014
Title cerebellum_WT_6 weeks_replicate 3
Sample type RNA
 
Source name cerebellum,WT,6 weeks
Organism Mus musculus
Characteristics tissue: cerebellum
genotype/variation: WT (129SvEv+FVB/N F1 hybrids)
age: 6 weeks
Treatment protocol not applicable
Growth protocol Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cRNA were prepared using the 3'IVT Express Kit (Ambion) from 100ng total RNA according to the manufacturer.
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HT MG-430 PM Array Plates. Plates were hybridized, washed and stained in the Affymetrix Gene Titan.
Scan protocol GeneChips were scanned using the Affymetrix Gene Titan instrument with AGCC 3.0 software.
Data processing The data were analysed using Bioconductor package 'affy' (1.28.0) under R-2.12.0. Background correction, normalization and probe summarization was performed with RMA.
 
Submission date Aug 13, 2014
Last update date Nov 04, 2014
Contact name Michael H. Walter
E-mail(s) [email protected]
Organization name University of Tuebingen
Department Department of Medical Genetics
Lab The Microarray Facility Tübingen
Street address Calwer Str. 7
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platform ID GPL11180
Series (1)
GSE60414 Potentiation of neurotoxicity in double mutant mice with Pink1 ablation and A53T-SNCA overexpression

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values.

Data table
ID_REF VALUE
1415670_PM_at 8.154207506
1415671_PM_at 10.52008353
1415672_PM_at 10.3656026
1415673_PM_at 8.026258741
1415674_PM_a_at 8.910747697
1415675_PM_at 8.139913675
1415676_PM_a_at 10.21068706
1415677_PM_at 8.087277898
1415678_PM_at 9.613682522
1415679_PM_at 10.35991673
1415680_PM_at 7.832334434
1415681_PM_at 9.670177067
1415682_PM_at 7.622428114
1415683_PM_at 9.726887496
1415684_PM_at 7.25306734
1415685_PM_at 7.187861368
1415686_PM_at 9.493039868
1415687_PM_a_at 11.61967588
1415688_PM_at 10.1517964
1415689_PM_s_at 9.131920874

Total number of rows: 45141

Table truncated, full table size 1159 Kbytes.




Supplementary file Size Download File type/resource
GSM1479313_E11R010b03.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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