|
| Status |
Public on Aug 01, 2014 |
| Title |
DFCs transfected with pEV and differentiated 1 |
| Sample type |
RNA |
| |
|
| Source name |
DFVs with pEV in differentiation medium
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dental progenitor/stem cells
|
| Treatment protocol |
After 48 h transfection and subconfluence cells at passage 6 were grown 3 days in the BMP2(10ng/ml) supplemented Osteogenesis Differentiation medium
|
| Growth protocol |
Cell were grown in DMEM medium (PAA, Pasching, Austria) and 10 % serum at 37°C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the RNeasy isolation kit (Qiagen,Hilden, Germany) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with 1biotin-labeled cRNA in a hybridization oven 640
|
| Scan protocol |
GeneTitan scanner (Affymetrix)
|
| Description |
Gene expression of DFCs after transfection with pEV and osteogenic differentiation with BMP2
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software
|
| |
|
| Submission date |
Jul 31, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Sandra Viale Bouroncle |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Regensburg
|
| Street address |
Franz-Josef-Strauß-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE59949 |
Expression data from human dental follicle cells |
|
