|
| Status |
Public on Jan 01, 2015 |
| Title |
YPS replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
YPD yeast cells_no_stress
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303 1-a
od600: 0.8
|
| Treatment protocol |
Stress caused by 20% (w/v) glucose or 1M Sorbitol during 30 minutes
|
| Growth protocol |
yeast cultures were kept in the exponential growth phase for 16h. OD600=0,8 cells were exposed to the osmotic stress conditions considered 20% (w/v) glucose (YP20), 1M sorbitol (YPS) or YPD (no stress conditions) during 30 min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA isolation using the protocol described in Carrasco et al., 2003
|
| Label |
Cy3
|
| Label protocol |
10 µg of total RNA reversed transcribed with SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| Channel 2 |
| Source name |
YPS yeast cells_1M_sorbitol
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303 1-a
od600: 0.8
|
| Treatment protocol |
Stress caused by 20% (w/v) glucose or 1M Sorbitol during 30 minutes
|
| Growth protocol |
yeast cultures were kept in the exponential growth phase for 16h. OD600=0,8 cells were exposed to the osmotic stress conditions considered 20% (w/v) glucose (YP20), 1M sorbitol (YPS) or YPD (no stress conditions) during 30 min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA isolation using the protocol described in Carrasco et al., 2003
|
| Label |
Cy5
|
| Label protocol |
10 µg of total RNA reversed transcribed with SuperScript II RTase (Invitrogen), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-label
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
Biological replicate 1/2
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jul 22, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Marcel·lí del Olmo |
| E-mail(s) |
[email protected]
|
| Phone |
34963543355
|
| Organization name |
Universitat de València
|
| Department |
Bioquímica i Biologia Molecular
|
| Street address |
Dr Moliner, 50
|
| City |
Burjassot (Valencia) |
| ZIP/Postal code |
46100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9825 |
| Series (1) |
| GSE59659 |
20% (w/v) Glucose osmotic stress (YP20) vs 1M Sorbitol osmotic stress (YPS) |
|
