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Sample GSM144129 Query DataSets for GSM144129
Status Public on Nov 14, 2006
Title No_abuse_8
Sample type RNA
 
Source name lymphoblast cell line
Organism Homo sapiens
Characteristics No abuse
Extracted molecule total RNA
Extraction protocol RNA was extracted from the cell lines using our standard procedures using an Invitrogen RNA purification kit (Invitrogen, Carlsbad, CA) according to the instructions provided by the manufacturer and stored in Ambion RNA storage solution at –80o
Label DIG-UTP
Label protocol Digoxigenin-UTP labeled cRNA was generated and amplified from 2 μg of total RNA from each sample using Applied Biosystems Chemiluminescent RT-IVT Labeling Kit v 1.0 (P/N 4340472) according to the manufacturer’s protocol (P/N 4339629).
 
Hybridization protocol Array hybridization was performed for 16 hrs at 55 oC. Chemiluminescence detection, image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
Scan protocol image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
Description No abuse in the Iowa Adoption Studies
Data processing Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software v 1.1 (P/N 4336391).
 
Submission date Nov 09, 2006
Last update date Nov 13, 2006
Contact name Gi-yung Ryu
E-mail(s) [email protected]
Organization name University of Iowa
Department Neurosurgery
Street address EMRB 200A
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL1426
Series (1)
GSE6264 Transcriptional Profiling of Subjects from the Iowa Adoption Studies

Data table header descriptions
ID_REF
VALUE Normalized signal
SIGNAL_RAW Raw signal
STDEV SDEV
S2N Signal/Noise
CV CV
FLAG generated by Applied Biosystems 1700 Chemiluminescent Microarray Analyzer

Data table
ID_REF VALUE SIGNAL_RAW STDEV S2N CV FLAG
226133 0.626644055388829 683.384064353255 118.505636351277 5.76667984236254 0.195409842715093 0
221463 132.451931227515 144444.901878999 2462.09436860356 58.6674920835486 0.0916761416942433 0
224689 0.169527960485051 184.878011147662 83.3291759496619 2.21864681896464 0.45963808463425 1024
223350 66.3516431433695 72359.5080459147 955.104200786652 75.7608520476795 0.0910395548162464 0
210244 0.104745113062786 114.229346740666 114.229346740666 -0.594408836267833 1.68475354926411 1
224206 0.0812901201024679 92.8907561256525 92.8907561256525 -0.621567403814923 1.61054390236502 1
226296 4.65402587414238 5075.4285309595 152.10249046816 33.3684775005176 0.0949319799802433 0
225542 0.917553619447373 1048.49456968274 91.4839746713909 11.4609643213352 0.11450545906098 0
226590 0.0782135650453765 85.295477565971 45.3869898029471 1.87929355827058 0.539685263477584 1024
225638 0.255517835583783 291.981915158312 291.981915158312 -0.0202637883723385 49.3491695885418 1
219335 0.282024571626781 307.560721829886 158.081464680718 1.94558370553484 0.521818082805864 0
220654 1.49023535061994 1625.16995412593 181.314908961131 8.9632450162955 0.143391465682799 0
235731 7.5174023976542 8198.07187143933 160.552779459905 51.0615381372867 0.0921819775216918 0
222802 0.163274817372479 178.058671973367 133.622064150391 1.33255441835536 0.75582521886451 0
222406 0.107838482314728 123.227744638967 123.227744638967 -0.97479629892602 1.02853180320467 1
227465 0.199302348531176 227.744107516914 87.6464529673402 2.59844066481251 0.391924815138253 0
218796 0.93064794420061 1063.45754089929 84.8696726696597 12.5304777012469 0.108937469892371 0
224249 0.152945320829232 174.771626364918 174.771626364918 0.0913710349675777 10.9446385556087 1
226419 0.240475730586292 274.793202616406 274.793202616406 -0.544547382404548 1.83788396696946 1
225134 0.511603674821988 557.927256520406 106.991988731733 5.21466385599513 0.211869122970193 0

Total number of rows: 33202

Table truncated, full table size 3094 Kbytes.




Supplementary data files not provided

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