|
| Status |
Public on Aug 01, 2014 |
| Title |
Thy1+SSEA1-_rep#2 |
| Sample type |
RNA |
| |
|
| Source name |
Cell culture
|
| Organism |
Mus musculus |
| Characteristics |
strain: CF-1
cell population: Thy1+SSEA1-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 5
Cell culture
replicate 2
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 08, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Tariq Rana |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, San Diego
|
| Department |
Pediatrics
|
| Lab |
Rana
|
| Street address |
9500 Gilman Drive, MC 0602
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE59175 |
Genome-wide RNAi screen reveals factors needed at the transition steps of induced reprogramming |
|
