|
Status |
Public on Jun 01, 2017 |
Title |
WAT - before bariatric surgery - rep16 |
Sample type |
RNA |
|
|
Source name |
Abdominal subcutaneous WAT
|
Organism |
Homo sapiens |
Characteristics |
tissue: Abdominal subcutaneous white adipose (WAT) gender: Female obesity status: before bariatric surgery
|
Treatment protocol |
Tissues were obtained by needle aspiration under local anasthesia
|
Growth protocol |
Human abdominal subcutaneous white adipose tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany) according to the instructions provided by the manufacturer
|
Label |
Biotin
|
Label protocol |
Total RNA was amplified and biotin labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
|
|
Hybridization protocol |
Biotinylated RNA was hybridized to Gene 1.1 ST Arrays, and then washed, stained and scanned using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
Scan protocol |
Standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA)
|
Description |
Gene expression profiling based on human abdominal subcutaneous white adipose tissue obtained by needle aspiration
|
Data processing |
Results were processed using the RMA method
|
|
|
Submission date |
Jul 02, 2014 |
Last update date |
Jun 01, 2017 |
Contact name |
Niklas Mejhert |
Organization name |
Karolinska Institutet
|
Street address |
Hälsovägen 7
|
City |
Stockholm |
ZIP/Postal code |
141 86 |
Country |
Sweden |
|
|
Platform ID |
GPL11532 |
Series (1) |
GSE59034 |
A Memory for Obesity in Adipose Tissue |
|