|
| Status |
Public on Jul 06, 2015 |
| Title |
wild-type mouse hippocampus 4 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed C57BL/B6N:C3B
tissue: hippocampus
|
| Growth protocol |
Hippocampi cere isolated from 4-month mice and flash frozen.Total RNA was prepared using Trizol (Invitrogen) according to the manufacturer's instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using Trizol (Invitrogen) and purified with the Rneasy Mini Kit (Quiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
In conjunction with the Affymetrix GeneChip WT Terminal Labeling Kit, the Ambion WT Expression Kits was designed to generate amplified and biotylated sense-strand DNA targets from the entire expressed genome
|
| |
|
| Hybridization protocol |
Samples were hynridized with Mouse Exon 1.0 ST Arrays (Affymetrix), washed and scaned using the GeneChip® Scanner 3000
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Raw data were processed with the Robust Multiarray Average (RMA) algorithm and values log transformed using Partek and GeneSptring softwares.
|
| |
|
| Submission date |
Jun 18, 2014 |
| Last update date |
Jul 06, 2015 |
| Contact name |
arbogast thomas |
| E-mail(s) |
[email protected]
|
| Phone |
+1 919-641-4968
|
| Organization name |
Duke University
|
| Department |
neurosciences
|
| Lab |
CHDM
|
| Street address |
300N Duke Street
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE58639 |
Transcriptomic expression analysis on hippocampus samples |
|
