|
| Status |
Public on Feb 26, 2015 |
| Title |
cont115 |
| Sample type |
RNA |
| |
|
| Source name |
control
|
| Organism |
Mus musculus |
| Characteristics |
strain background: B6C3F1
gender: male
age: 7 days
treated with: deionized water alone (dH2O, control)
tissue: liver
|
| Treatment protocol |
Dichloroacetic acid (CAS 79-43-6, Aldrich, Milwaukee, WI) and phenobarbital (PB) (CAS 50-06-6, Sima Chemical Co., St.Louis, MO) were administered in the drinking water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 20 mg of archived frozen liver tissue was homogenized in RNAzol®RT ( Molecular Research Center, Cincinnati, Ohio) using ceramic beads in the Precellys® 24 homogenizer (Bertin Technologies, Villeurbanne, France).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was produced from 200ng total RNA using the Illumina TotalPrep RNA Amplification kit (cat# AMIL1791).
|
| |
|
| Hybridization protocol |
Samples were hybridized on to (Mouse Ref-8 V2.0 Expression BeadChip) which a multi sample format allowing 8 samples per chip
|
| Scan protocol |
Arrays were scanned on the iScan module and raw data (.idat files) were obtained using the illumina iScan control software Operating Software (version v3.3.28) and analyzed by GenomeStudio® (Illumina Inc.)
|
| Description |
GRC225-EC115; The GRC# is the study# and the EC# is the animal number
|
| Data processing |
For analysis of microarray data, raw intensities were quantile normalized from the Illumina Genome Studio® (Illumina Inc.).
|
| |
|
| Submission date |
Jun 17, 2014 |
| Last update date |
Feb 26, 2015 |
| Contact name |
Susan Hester |
| E-mail(s) |
[email protected]
|
| Phone |
919-541-1320
|
| Organization name |
US EPA
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL15097 |
| Series (1) |
| GSE58585 |
Tumorigenic carryover effects of early life exposure to dichloroacetic acid in male B6C3F1 mice |
|
