|
| Status |
Public on Oct 21, 2006 |
| Title |
NRMRL test replicate03, EPA1UVHK batch072104 430B chip |
| Sample type |
RNA |
| |
|
| Source name |
mouse small intestinal cell culture, 5 hour infection
|
| Organism |
Mus musculus |
| Characteristics |
MICcl2 cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol procedure
|
| Label |
Streptavidin Phycoerythrin (SAPE)
|
| Label protocol |
Affymetrix SOP, EukGE-WS2
|
| |
|
| Hybridization protocol |
MAS 5.0 software EukGE-WS1
|
| Scan protocol |
MAS 5.0 with model 4500 scanner
|
| Description |
None
|
| Data processing |
Microarray Suite (MAS) 5.0 software, target signal scaling 500; normalization 1; Alpha1 0.05; Alpha2 0.065; Tau 0.015; Gamma1L and 1H 0.0045; Gamma 2L and 2H 0.06; and Pertubation 1.1.
|
| |
|
| Submission date |
Oct 18, 2006 |
| Last update date |
Oct 20, 2006 |
| Contact name |
Samuel Hayes |
| E-mail(s) |
[email protected]
|
| Phone |
513-569-7514
|
| Organization name |
United States Environmental Protection Agency
|
| Department |
MS-387
|
| Lab |
NRMRL/WSWRD
|
| Street address |
26 W. Martin Luther King Dr.
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45268 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE6065 |
Murine host cell response to Aeromonas infection |
|
