|
| Status |
Public on May 28, 2014 |
| Title |
case-6_Germline DNA |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
gender: M
age: 65
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was obtained using proteinase K/RNAse treatment and phenol-chloroform extraction according to standard procedures.
|
| Label |
Cy3
|
| Label protocol |
Patient’s DNA and reference DNA samples were labeled by random priming with either, Cy3-dUTP or Cy5-dUTP using the Agilent Genomic DNA Labeling Kit PLUS
|
| |
|
| Channel 2 |
| Source name |
pooled healthy donor
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was obtained using proteinase K/RNAse treatment and phenol-chloroform extraction according to standard procedures.
|
| Label |
Cy5
|
| Label protocol |
Patient’s DNA and reference DNA samples were labeled by random priming with either, Cy3-dUTP or Cy5-dUTP using the Agilent Genomic DNA Labeling Kit PLUS
|
| |
|
| |
| Hybridization protocol |
Microarrays were hybridized using the Sure Hyb chamber in a rotisserie oven at 65°C for 40 h.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version11.0.1.1).
|
| Data processing |
Analysis and visualization were performed with DNA Analytics software (version 4.081, Agilent)
|
| |
|
| Submission date |
May 27, 2014 |
| Last update date |
May 28, 2014 |
| Contact name |
Akinobu Ota |
| E-mail(s) |
[email protected]
|
| Organization name |
Aichi Medical University School of Medicine
|
| Department |
Biochemistry
|
| Street address |
1-1 Yazakokarimata
|
| City |
nagakute |
| State/province |
Aichi |
| ZIP/Postal code |
4801195 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9777 |
| Series (1) |
| GSE58000 |
Array CGH of cutaneous neurofibromas (cNFs) from NF1 patients |
|
