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Status
Public on Feb 01, 2007
Title
UCB-MSC_UCB2_rep2B
Sample type
RNA
Source name
human umbilical cord blood-derived mesenchymal stem cell population 2, replicate 2
Organism
Homo sapiens
Characteristics
Tissue: umbilical cord blood-derived mesenchymal stem/stromal cell
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/ Coriell
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Description
none
Data processing
RMA method implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected]
Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php
Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL97
Series (1)
GSE6029
Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions
ID_REF
VALUE
RMA expression estimate
Data table
ID_REF
VALUE
200000_s_at
8.67875852128913
200001_at
10.6069663230247
200002_at
11.0525642228058
200003_s_at
11.3422938144352
200004_at
10.8241802243804
200005_at
9.84496629143448
200006_at
10.6198364672255
200007_at
10.3906695928849
200008_s_at
9.59727210454116
200009_at
10.7137968350173
200010_at
10.8094063648819
200011_s_at
8.36930392666163
200012_x_at
11.4169206432497
200013_at
11.5049288369592
200014_s_at
9.45887210759021
200015_s_at
9.96748735058318
200016_x_at
10.7862022914687
200017_at
11.1865716243912
200018_at
11.6682522856087
200019_s_at
11.0633671708023
Total number of rows: 22645 Table truncated, full table size 602 Kbytes .Supplementary file
Size
Download
File type/resource
GSM139905.CEL.gz
2.9 Mb
(ftp) (http)
CEL